Incidence of Listeria monocytogenes in Meat Product Samples by Real-Time PCR
نویسندگان
چکیده
منابع مشابه
Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR.
We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detecti...
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A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2.
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Listeria monocytogenes is a bacterial pathogen that can invade the central nervous system (CNS), causing meningoencephalitis and brain abscesses. The diagnosis of CNS listeriosis, based on the isolation of the bacteria in the cerebrospinal fluid (CSF), can be difficult because of previous antibiotic treatment and a low number of bacteria in the CSF. To improve the sensitivity of microbiological...
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8 1 Département de Microbiologie, Hôpital Necker-Enfants Malades, Assistance Publique9 Hôpitaux de Paris, Université Paris Descartes, France 10 2 Centre National de Référence des Listeria, World Health Organization Collaborating Centre 11 for Foodborne Listeriosis, Institut Pasteur, Paris, France. 12 3 Actual Adress: Laboratoire de Microbiologie-Hygiène, Centre Hospitalier de Versailles, Le 13 ...
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ژورنال
عنوان ژورنال: Modern Chemistry and Applications
سال: 2015
ISSN: 2329-6798
DOI: 10.4172/2329-6798.1000155